Experiment 1: Culture Transfer Techniques

-
 
First microbiology lab experiment class with DR. Adelene Song...

Assalamualaikum.... For my first microbiology lab class with DR. Adelene Song was actually fun and interesting because I got learned some techniques how to transfer of microorganisms for subculturing, how to correctly sterilize inoculating instruments in the flame of in bunsen burner. MR.Hussain  was taught us the concept of aseptic technique and the procedures necessary for successful subculturing of microorganisms. DR.Adelene also taught us how to write a proper lab report and the laboratory skills. It is very helpful to us.

 Experiment 1: Culture Transfer Techniques

   Microbiology is the study of microscopic organisms, those being unicellular (single cell), multicellular (cell colony), or acellular(lacking cells). Microbiology encompasses numerous sub-disciplines including virology, mycology, parasitology, and bacteriology. The microbiologist separates these mixed populations into individual species for study. Microorganisms are transferred from one medium to another by subculturing. This tehnique is of basic importance and is used routinely in preparing and maintaining stock cultures, as well as in microbiological test procedures. A culture containing a single unadulterated species of cells is called pure culture.To isolate and study microorganisms in pure culture, the microbiologist requires basic laboratory apparatus and the applicationof specific techniques. Microorganisms are always present in the air and on laboratory surfaces, benches, and equipment. These ambient microorganisms can serve as a source of external contamination and interfere with experimental results unless proper aseptic techniques are used during subculturing.

   Aseptic technique is a fundamental and important laboratory skill in the field of microbiology. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. Proper aseptic tecnique prevents contamination of cultures from foreign bacteria inherent in the environment. Forms of culture media, broth tubes are tubes containing a liqiud medium. Slants tubes are tubes containing a nutrient medium plus a solidifying agent, agar-agar. The medium has been allowed to solidify at an angle in order to get a flat inoculatingsurface. Stab tubes (deeps) are tubes of hardened agar medium which are inoculated by "stabbing" the inoculum into the agar.

                                                     from Serratia marcescens to nutrient medium


                                                 
from culture "A" to nutrient medium

from culture "B" to nutrient medium 

 
 

Comments

Post a Comment

Popular posts from this blog

experiment 4: Microscopic examination of stained cell preparation and experiment 5: Microscopic examination of living microorganisms using a Hanging-Drop Preparation or a Wet Mount

-
Image
 Assalamualaikum and good morning everyone..... how are you guys?.😃😃😃.. so now Im going to share with you about my experience when I did my forth anf fifth experiment which are forth experiment about Microscopic examination of stained cell preparations and fifth experiment about Microscopic examination of living microorganisms using a Hanging-drop preparation or a Wet mount. First and foremost, after I was entered to the class, DR Adelene taught about the parts of microscope, different types of microscopes, the function of each parts of microscopes.  DR also talked about the magnifications, resolving power or resolution and so on. The experiment that we did this week are more interesting than last week because we saw microorganisms under microscope.😁😁😁 Mr Hussain also taught us how to clean the objective lens, how to hold the microscope and gave some demo. For the fifth experiment, Mr Zainuddin was explained about the experiment and taught us how to do the experiment. For an exam
Read more

Experiment 6: Preparation of bacterial smear, Experiment 7: Simple staining and Experiment 8: Negative staining

-
Image
      Assalamualaikum and every good evening to everyone that read my post... The experiment I did in this week which is sixth week of basic technique of microbiology have three experiment. First is preparation of bacterial smears, next is simple staining and the last one is negative staining. Bacterial smears must be prepared prior to the execution of any of the staining technique. Bacterial cells are usually colorless because cytoplasm, for the most part, is transparent. Since the bacteria are colorless, it is almost essential to add a stain to make the bacteria more visible. Once stained, cell morphology can be observed. In simple staining, the bacterial smear is stained with a single reagent, which produces a distinctive contrast between the organism and its background. In negative staining requires the use of an acidic stain such as India ink or nigrosin. The acidic stain, with its negatively charged chromogen, will not penetrate the cells because of the negative charge on the sur
Read more