Experiment 1: Culture Transfer Techniques

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First microbiology lab experiment class with DR. Adelene Song...

Assalamualaikum.... For my first microbiology lab class with DR. Adelene Song was actually fun and interesting because I got learned some techniques how to transfer of microorganisms for subculturing, how to correctly sterilize inoculating instruments in the flame of in bunsen burner. MR.Hussain  was taught us the concept of aseptic technique and the procedures necessary for successful subculturing of microorganisms. DR.Adelene also taught us how to write a proper lab report and the laboratory skills. It is very helpful to us.

 Experiment 1: Culture Transfer Techniques

   Microbiology is the study of microscopic organisms, those being unicellular (single cell), multicellular (cell colony), or acellular(lacking cells). Microbiology encompasses numerous sub-disciplines including virology, mycology, parasitology, and bacteriology. The microbiologist separates these mixed populations into individual species for study. Microorganisms are transferred from one medium to another by subculturing. This tehnique is of basic importance and is used routinely in preparing and maintaining stock cultures, as well as in microbiological test procedures. A culture containing a single unadulterated species of cells is called pure culture.To isolate and study microorganisms in pure culture, the microbiologist requires basic laboratory apparatus and the applicationof specific techniques. Microorganisms are always present in the air and on laboratory surfaces, benches, and equipment. These ambient microorganisms can serve as a source of external contamination and interfere with experimental results unless proper aseptic techniques are used during subculturing.

   Aseptic technique is a fundamental and important laboratory skill in the field of microbiology. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. Proper aseptic tecnique prevents contamination of cultures from foreign bacteria inherent in the environment. Forms of culture media, broth tubes are tubes containing a liqiud medium. Slants tubes are tubes containing a nutrient medium plus a solidifying agent, agar-agar. The medium has been allowed to solidify at an angle in order to get a flat inoculatingsurface. Stab tubes (deeps) are tubes of hardened agar medium which are inoculated by "stabbing" the inoculum into the agar.

                                                     from Serratia marcescens to nutrient medium


                                                 
from culture "A" to nutrient medium

from culture "B" to nutrient medium 

 
 

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